chymotrypsin /kī′mōtrip″sin/ [Gk, chymos + tryein, to rub, pepsin, digestion] , 1. a proteolytic enzyme produced by the pancreas that catalyzes the hydrolysis of casein and gelatin. 2. a yellow crystalline powder prepared from an extract of ox pancreas that is used in treating digestive disorders in which the enzyme is present in less than normal amounts or is totally lacking.