Allen-Doisy test [Edgar Allen, U.S. endocrinologist, 1892–1943; Edward Doisy, U.S. biochemist and Nobel laureate, 1893–1986] , a once-common bioassay test for estrogen and gonadotropins in which ovariectomized mice or rats were injected with an estrogenic substance. The appearance of cornified cells on vaginal smears and the disappearance of leukocytes constituted a positive reaction.
