hybridization /hī′bridīzā″shən/ , 1. the process of producing hybrids by crossbreeding. 2. the process of combining single-stranded nucleic acids from different sources to form stable, double-stranded molecules. The technique involves fragmentation and separation of the source nucleic acids by heating, followed by recombination through cooling. The resulting hybrids can be DNA-DNA, DNA-RNA, or RNA-RNA duplexes.
